|Title: Purification of Cellulase obtained from Tomato fruits (Lycopersicon lycopersicum (L.) Karst) deteriorated by Aspergillus Flavus Linn|
|Author(s): Ajayi A. A, Adebiyi M. O, Olasehinde G. I, Olutiola P. O|
|Pages: 1-3||Paper ID: 104205-9393-IJBAS-IJENS||Published: October, 2010|
Abstract: Tomato fruits infected by Aspergillus flavus Linn produced proteins with cellulolytic activity. The enzyme was partially purified by Ammonium Sulphate Precipitation, Gel filtration and ion-exchange chromatography. Three peaks of absorption A, B and C were obtained. Peak B had Cellulase activity with molecular weight of approximately 30,200 Daltons while Peaks A and C lacked Cellulase activity. Elution of components of Peak B on CM Sephadex C-25 produced four peaks of absorption designated Ba, Bb, Bc and Bd. Only components of Peaks Bb and Bc possessed Cellulase activity. Purification folds of approximately 80 and 81 were obtained for components of Peaks Bb and Bc respectively for Cellulase of A. flavus. The apparent Km values for the hydrolysis of carboxymethylcellulose by A.flavus Cellulase fractions, Bb and Bc were approximately 16.7 and 15.4mg/ml respectively. The partially purified enzyme preparations obtained from A.flavus during the deterioration of tomato fruits caused tissue maceration and cellular death. This result can be very useful in splitting and solubilization of pectic substances and pathogenicity.
|Keywords: Purification, Cellulase, Tomato fruits, Aspergillus flavus linn .|
|Full Text (.pdf) | 199 KB|
|Title: The Presence of Arsenic as Heavy Metal Contaminant on Salmon : a Risk Assessment|
|Author(s): Titik Budiati|
|Pages: 4-7||Paper ID: 103205-6565-IJBAS-IJENS||Published: October, 2010|
Abstract: Salmon is a kind of fish which has good nutrition for human but it can be contaminated by heavy metal such as arsenic. In risk assessment , the provisional tolerable weekly intake (PTWI) for inorganic arsenic is 15 µg/kg b.w./week and the organic forms of arsenic present in sea foods need different consideration from the inorganic arsenic in water. There are no reports of toxicity in man or animals from the consumption of organoarsenicals in seafood. Organic arsenic compounds such as arsenobetaine and arsenocholine seem not to be converted to inorganic arsenic in vivo and not genotoxic in mammalian cells in vitro. Therefore, arsenobetaine and arsenocholine from fish and sea food consumption is not considered to represent a significant health risk.
|Keywords: Salmon, arsenic, risk assessment.|
|Full Text (.pdf) | 300 KB|
|Title: Age of the Mfamosing Limestone, Calabar Flank, South Eastern, Nigeria|
|Author(s): Essien, N. U., Ufot, D. O.|
|Pages: 8-19||Paper ID: 107105-6464-IJBAS-IJENS||Published: October, 2010|
Abstract: Palynological analyses of outcrop samples and cores from two (2) wells were used in the determination of the age of the Mfamosing Limestone. Quantitative palynological analyses of the shaly intercalations in outcrop sections and the shales immediately overlying this limestone formation reveals the presence of index Albian-Cenomanian palynomorphs such as Classopollis jardinei, Ephedripites procerus, Ephedripites striagatus, Elaterosporites protensus and Elaterosporites sp. The palynological analyses points to a Mid Albian – Early Cenomanian age for the Mfamosing Limestone.
|Keywords: Mfamosing Limestone, Age, Palynomorph, Albian - Cenomanian.|
|Full Text (.pdf) | 637 KB|
|Title: A Study on the Level of Ethics at a Malaysian Private Higher Learning Institution: Comparison between Foundation and Undergraduate Technical-based Students|
|Author(s): Khalidah Khalid Ali, Rohani Salleh, Mashitah Sabdin|
|Pages: 20-30||Paper ID: 1086-04-05-7575-IJBAS-IJENS||Published: October, 2010|
Abstract: The environment and socialization process to which students are exposed remain as important factors contributing to academic success and character building. The Malaysian educational system, at both primary and tertiary levels have consistently emphasized on the importance of nurturing moral values and ethics among students. However, ethical problems among the Generation Y are often highlighted, be it at home or the workplace. As the general well being and prosperity of a nation depend largely on the internalization of good moral values among members of the community, an exploratory study was initiated to compare the level of ethics possessed by foundation and final year Engineering and Technology students at a higher learning institution in Malaysia. This paper aims to report on the research findings. A questionnaire was developed to measure five universally accepted moral values: Integrity, Honesty, Respect for humans and non-humans, Self Control and Courage. The comparative results of the study will give an indication whether the environment of the University has significantly contributed in shaping the ethical values of the students prior to joining the labour market. The findings may also show the extent of the contribution and effectiveness of ethics related subjects in developing the moral values of students.
|Keywords: Ethics, Environment, Malaysia, Higher Learning Institution, Foundation, Engineering, Technology, Integrity, Honesty, Respect, Self Control, Courage.|
|Full Text (.pdf) | 457 KB|